|Race||Human/Tengu Clan Member|
|Family||Konoka Konoe (Grandmother)|
Setsuna Sakurazaki (Grandmother)
|First Appearance||Chapter 95|
Isana is a 12 year old girl that looks like a younger version of Setsuna, but with more of Konoka's lighter facial features. In her first appearance she is shown wearing a school uniform.
She's comparative quiet to Honoka, sharing a similar personality to her grandmother Setsuna.
Isana appears along with Honoka to capture Touta and take him to Fate, who they are aligned with. She uses the Shinmeiryuu sword style to keep him off balance long enough for Honoka to imprison him with a water spell, but Touta used his Magic Cancel to free himself and strip the girls of their magical school uniforms.
Honoka and Isana are twin sisters who have a easygoing sibling relationship.
- Isana and Honoka made their first appearance in Negima!.
- While Isana and Honoka refer to Touta as "Onii-sama" (meaning roughly "respectful Big brother"), the honorific is not used as a indication of blood relation, but rather, a term to refer to one's elders (in English, a counterpart for such a term does not culturally exist), similar to how one could call non-family people "uncle" or "aunty".